A single-cell transcriptome atlas of the ageing Drosophila brain

Experiment ID:SRP125768
Technology:scRNA-Seq
Description:Overall design: scRNA-seq (10x Genomics CHROMIUM Single Cell 3' Solution V2 Chemistry and Drop-seq) of adult brains from Drosophila melanogaster at various ages; SMART-seq2 on FAC-sorted R23E10-Gal4 positive neurons of adult brains from Drosophila melanogaster; Adapted SMART-seq2 on FAC-sorted R23E10-Gal4 positive neurons of adult brains from Drosophila melanogaster; CEL-seq2 on FAC-sorted R23E10-Gal4 positive neurons of adult brains from Drosophila melanogaster; Bulk RNA-seq of adult brains from Drosophila melanogaster; ATAC-seq of young (0 days) and old (50 days) adult brains from Drosophila melanogaster
Cell count:56,902
Source:SRP125768  SRA

List of assays

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entries

Library ID
Identifier of the RNA-Seq library
Cell type ID
ID of the cell type of the sample
Cell type name
Name of the cell type of the sample
Cell type author annotation
Free text annotation of cell type as provided by authors
Anat. entity ID
ID of the anatomical localization of the sample
Anat. entity name
Name of the anatomical localization of the sample
Anat. entity author annotation
Free text annotation of anatomical localization of the sample as provided by authors
Stage ID
ID of the developmental and life stage of the sample
Stage name
Name of the developmental and life stage of the sample
Stage author annotation
Free text annotation of the developmental and life stage of the sample as provided by authors
Sex
Annotation of the sex of the sample
Strain
Annotation of the strain of the sample
Time
Free text annotation of the time of sampling as provided by authors
Time unit
Unit for the time of sampling as provided by authors
Species
Physiological status
Physiological status of the organism at time of sampling
Technology
Sequencing platform
Sequenced transcript part
Possible values are: full length, all parts of the transcript are sequenced; 3': only the 3' end of the transcript is sequenced; 5': only the 5' end of the transcript is sequenced.
Fractionation
Possible values are: cell, transcripts are extracted from the cell; nuclei, transcripts are extracted from the nucleus.
Fragmentation
Size of the RNA fragmentation
Run sequencing type
Paired-end or single-read run
Total read count
Total number of reads for the library.
Mapped read count
Number of reads that could be mapped to the transcriptome.
Total UMI count
Total number of individual RNA molecules (UMI) for the annotated sample. Only applicable for libraries producing UMIs.
Mapped UMI count
Number of UMIs that could be mapped to the transcriptome. Only applicable for libraries producing UMIs.
Distinct rank count
When performing a fractional ranking of the genes in the annotated sample, based on their expression level, number of distinct ranks observed, to have a value of the power for distinguishing expression levels. Used as a weight to compute a weighted mean rank accross samples for each gene and compute expression scores in Bgee.
Max rank
When performing a fractional ranking of the genes in the annotated sample, based on their expression level, maximum rank attained in the sample. Used to normalize ranks accross samples and compute expression scores in Bgee.
Link to processed expression values
See the processed expression value results for this assay
SRX4084634NAUBERON:0000955 brainWholeBrainFBdv:00007076 day 1 of adulthood (fruit fly)1 day adult flymixedDGRP-551NANADrosophila melanogasterNAIlluminaNextSeq 500FULL_LENGTHCELL50single29364492162697001095112904Browse results
SRX4084635NAUBERON:0000955 brainWholeBrainFBdv:00007076 day 1 of adulthood (fruit fly)1 day adult flymixedDGRP-551NANADrosophila melanogasterNAIlluminaNextSeq 500FULL_LENGTHCELL50single1937402913831337001363314302.5Browse results
SRX4084636NAUBERON:0000955 brainWholeBrainFBdv:00007076 day 1 of adulthood (fruit fly)1 day adult flymixedwild-typeNANADrosophila melanogasterNAIlluminaNextSeq 500FULL_LENGTHCELL50single45136963334214001162613268Browse results
SRX4084637NAUBERON:0000955 brainWholeBrainFBdv:00007076 day 1 of adulthood (fruit fly)1 day adult flymixedwild-typeNANADrosophila melanogasterNAIlluminaNextSeq 500FULL_LENGTHCELL50single35525752584697001192413418Browse results
SRX3426759CL:0000210 photoreceptor cellPhotoreceptorsUBERON:0000955 brainbrainFBdv:00007075 day 0 of adulthood (fruit fly)less than 8 HoursNADGRP-551NANADrosophila melanogasterNA10X Genomics V2NextSeq 500THREE_PRIMECELL0paired9599886770038300001869298Browse results
SRX3426759CL:0000381 neurosecretory neuronNAUBERON:0000955 brainbrainFBdv:00007075 day 0 of adulthood (fruit fly)less than 8 HoursNADGRP-551NANADrosophila melanogasterNA10X Genomics V2NextSeq 500THREE_PRIMECELL0paired9599886770038300002219471.5Browse results
SRX3426759CL:0002319 neural cellNAUBERON:0000955 brainbrainFBdv:00007075 day 0 of adulthood (fruit fly)less than 8 HoursNADGRP-551NANADrosophila melanogasterNA10X Genomics V2NextSeq 500THREE_PRIMECELL0paired959988677003830000236712155Browse results
SRX3426759CL:2000029 central nervous system neuronPoxn (-expressing neurons)UBERON:0000955 brainbrainFBdv:00007075 day 0 of adulthood (fruit fly)less than 8 HoursNADGRP-551NANADrosophila melanogasterNA10X Genomics V2NextSeq 500THREE_PRIMECELL0paired9599886770038300002649766Browse results
SRX3426759FBbt:00001258 subperineurial glial cell (Drosophila)Subperineurial_gliaUBERON:0000955 brainbrainFBdv:00007075 day 0 of adulthood (fruit fly)less than 8 HoursNADGRP-551NANADrosophila melanogasterNA10X Genomics V2NextSeq 500THREE_PRIMECELL0paired9599886770038300000Browse results
SRX3426759FBbt:00003702 optic lobe neuron (Drosophila)T4/T5 (neuron, optic lobe)UBERON:0000955 brainbrainFBdv:00007075 day 0 of adulthood (fruit fly)less than 8 HoursNADGRP-551NANADrosophila melanogasterNA10X Genomics V2NextSeq 500THREE_PRIMECELL0paired9599886770038300002069434.5Browse results
SRX3426759FBbt:00003718 lamina monopolar neuron (Drosophila)NAUBERON:0000955 brainbrainFBdv:00007075 day 0 of adulthood (fruit fly)less than 8 HoursNADGRP-551NANADrosophila melanogasterNA10X Genomics V2NextSeq 500THREE_PRIMECELL0paired9599886770038300002669654Browse results
SRX3426759FBbt:00003719 lamina monopolar neuron L1 (Drosophila)L1UBERON:0000955 brainbrainFBdv:00007075 day 0 of adulthood (fruit fly)less than 8 HoursNADGRP-551NANADrosophila melanogasterNA10X Genomics V2NextSeq 500THREE_PRIMECELL0paired959988677003830000448087Browse results
SRX3426759FBbt:00003721 lamina monopolar neuron L3 (Drosophila)L3UBERON:0000955 brainbrainFBdv:00007075 day 0 of adulthood (fruit fly)less than 8 HoursNADGRP-551NANADrosophila melanogasterNA10X Genomics V2NextSeq 500THREE_PRIMECELL0paired959988677003830000778280Browse results
SRX3426759FBbt:00003741 lamina wide-field cell (Drosophila)Lawf1UBERON:0000955 brainbrainFBdv:00007075 day 0 of adulthood (fruit fly)less than 8 HoursNADGRP-551NANADrosophila melanogasterNA10X Genomics V2NextSeq 500THREE_PRIMECELL0paired9599886770038300002119487.5Browse results
SRX3426759FBbt:00003743 centrifugal neuron C2 (Drosophila)C2UBERON:0000955 brainbrainFBdv:00007075 day 0 of adulthood (fruit fly)less than 8 HoursNADGRP-551NANADrosophila melanogasterNA10X Genomics V2NextSeq 500THREE_PRIMECELL0paired9599886770038300001098660Browse results
SRX3426759FBbt:00003744 centrifugal neuron C3 (Drosophila)C3UBERON:0000955 brainbrainFBdv:00007075 day 0 of adulthood (fruit fly)less than 8 HoursNADGRP-551NANADrosophila melanogasterNA10X Genomics V2NextSeq 500THREE_PRIMECELL0paired9599886770038300001859300Browse results
SRX3426759FBbt:00003767 distal medullary amacrine neuron (Drosophila)Dm8/Dm11UBERON:0000955 brainbrainFBdv:00007075 day 0 of adulthood (fruit fly)less than 8 HoursNADGRP-551NANADrosophila melanogasterNA10X Genomics V2NextSeq 500THREE_PRIMECELL0paired9599886770038300001559215.5Browse results
SRX3426759FBbt:00003776 medullary intrinsic neuron Mi1 (Drosophila)Mi1UBERON:0000955 brainbrainFBdv:00007075 day 0 of adulthood (fruit fly)less than 8 HoursNADGRP-551NANADrosophila melanogasterNA10X Genomics V2NextSeq 500THREE_PRIMECELL0paired9599886770038300001348829.5Browse results
SRX3426759FBbt:00003788 transmedullary neuron (Drosophila)Tm1/TmY8UBERON:0000955 brainbrainFBdv:00007075 day 0 of adulthood (fruit fly)less than 8 HoursNADGRP-551NANADrosophila melanogasterNA10X Genomics V2NextSeq 500THREE_PRIMECELL0paired9599886770038300002309557Browse results
SRX3426759FBbt:00003797 transmedullary neuron Tm9 (Drosophila)Tm9UBERON:0000955 brainbrainFBdv:00007075 day 0 of adulthood (fruit fly)less than 8 HoursNADGRP-551NANADrosophila melanogasterNA10X Genomics V2NextSeq 500THREE_PRIMECELL0paired95998867700383000046110430Browse results